EpiTect Whole Bisulfitome Kit

EpiTect Whole Bisulfitome Kit

Cat. No. / ID: EpiTect Whole Bisulfitome Kit  

Price : $0 ~ $22,500

Unit

For whole genome amplification of bisulfite converted DNA for PCR analyses

  • High yield of bisulfite converted DNA for multiple PCRs
  • Representative amplification from bisulfite converted DNA
  • Convenient one-step reaction setup

The EpiTect Whole Bisulfitome Kit provides DNA polymerase, buffers, and reagents for amplification of the entire bisulfite converted genome — the bisulfitome — using multiple displacement amplification technology (MDA). The EpiTect Whole Bisulfitome Kit has been specially developed for the amplification of DNA after conversion with EpiTect Bisulfite Kits. It uses proven REPLI-g technology and has been adapted to the specific requirements of bisulfite converted DNA (smaller DNA fragment size and altered nucleotide composition due to bisulfite conversion), while maintaining the converted sequence representation. Typical DNA yields from the EpiTect Whole Bisulfitome Kit are 1–3 μg per reaction, sufficient for up to 300 PCR analyses from just a single EpiTect bisulfite reaction.

The EpiTect Whole Bisulfitome Kit is the ideal tool to provide sufficient DNA for all downstream methylation analyses, whenever bisulfite converted DNA is limited. The amplification reaction produces enough DNA for 100–300 real-time PCR assays (e.g., using EpiTect MethyLight PCR Kits) or end-point PCRs (e.g., using EpiTect MSP Kits), estimating 10 ng of amplified DNA per reaction.

REPLI-g Midi DNA Polymerase, EpiTect WBA Reaction Buffer, nuclease-free water for 25 whole bisulfitome amplification reactions